Difference between revisions of "Part:BBa K3630029"
 
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<partinfo>BBa_K3630029 short</partinfo>
 
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Constitutively promote ferritin to accumulate Fe ion and make bacteria become positive change. The ferritin that we make has already been mutated and combine with iBox-PAK4. iBox- PAK4 can crystalization the protein and enhance the accumulation of Fe ion to increase the magnetic ability.
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As our BREAC is a microorganism that could tip the balance of the aquarium ecosystem, its removal is another necessary problem that we have to address. Aquariums usually have a filter to clean the water. However, it cannot guarantee the removal of our bacteria. Hence, our design has evolved to include to have the third feature of the project, that is, the magnetization system. When a magnetic field is applied, E. coli can be magnetized and stick on the magnet and it could be cleared.
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Constitutively promotes ferritin expression. The ferritin has been linked to iBox-PAK4 which forms crystal structures and enhances the bacteria's sensitivity to magnetic force.
  
 
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       <img src="https://static.igem.org/mediawiki/parts/6/61/T--UM_Macau--Ferritin.png" class= "center" width="800"
 
       <img src="https://static.igem.org/mediawiki/parts/6/61/T--UM_Macau--Ferritin.png" class= "center" width="800"
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<p style="text-align: justify; font-size: 14px; font-family: MuliLight; color: black; margin-left: auto; margin-right: auto;"><b>Figure.</b>Magnetization expression construction</p>
 
<p style="text-align: justify; font-size: 14px; font-family: MuliLight; color: black; margin-left: auto; margin-right: auto;"><b>Figure.</b>Magnetization expression construction</p>
 
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Latest revision as of 20:08, 27 October 2020


Enhanced ferritin expression system

As our BREAC is a microorganism that could tip the balance of the aquarium ecosystem, its removal is another necessary problem that we have to address. Aquariums usually have a filter to clean the water. However, it cannot guarantee the removal of our bacteria. Hence, our design has evolved to include to have the third feature of the project, that is, the magnetization system. When a magnetic field is applied, E. coli can be magnetized and stick on the magnet and it could be cleared.

Constitutively promotes ferritin expression. The ferritin has been linked to iBox-PAK4 which forms crystal structures and enhances the bacteria's sensitivity to magnetic force.

HTML img Tag

Figure.Magnetization expression construction

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]