Difference between revisions of "Part:BBa K3484002"
 
(One intermediate revision by the same user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K3484002 short</partinfo>
 
<partinfo>BBa_K3484002 short</partinfo>
  
−
This part encodes an intein-mediated T3 biosensor with enhanced GFP (eGFP) as reporter (IMT3_eGFP). This part was an intermediate sensor to later on develop the IMT3_sfGFP (<html><a href="https://parts.igem.org/Part:BBa_K3484001">BBa_K3484001</a></html>). Visit its page to see the caracteritazationa and final design.  
+
This part encodes an intein-mediated T3 biosensor with enhanced GFP (eGFP) as a reporter (IMT3_eGFP). This part was an intermediate sensor to later on develop the IMT3_sfGFP (<partinfo>BBa_K3484000</partinfo>). Visit its page to see the characterization and final design.  
  
  

Latest revision as of 20:28, 27 October 2020


Intein-mediated T3(THRB) → eGFP

This part encodes an intein-mediated T3 biosensor with enhanced GFP (eGFP) as a reporter (IMT3_eGFP). This part was an intermediate sensor to later on develop the IMT3_sfGFP (BBa_K3484000). Visit its page to see the characterization and final design.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 174
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 664
    Illegal NgoMIV site found at 2167
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1579