Difference between revisions of "Part:BBa K3365017:Design"
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Little ghost (Talk | contribs) (→References) |
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===Source=== | ===Source=== | ||
| − | + | The ligation of BBa_K3365003, BBa_K3365054 and BBa_K3365002 by Overlap PCR. | |
===References=== | ===References=== | ||
| + | |||
| + | [1] Guzman LM, Belin D, Carson MJ, Beckwith J. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriol. 1995 Jul;177(14):4121-30. | ||
| + | [2] Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim WA. Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression. Cell. 2013 Feb 28;152(5):1173-83. | ||
| + | [3] Vigouroux A, Bikard D. CRISPR Tools To Control Gene Expression in Bacteria. Microbiol Mol Biol Rev. 2020 Apr 1;84(2):e00077-19. | ||
Latest revision as of 15:44, 27 October 2020
pBAD upstream of eGFP with inhibition unit containing lure3
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1053
Illegal XhoI site found at 1062 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 74
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 56
Design Notes
The restriction cutting sites are added to the fragment during PCR for easy ligation to the backbone.
Source
The ligation of BBa_K3365003, BBa_K3365054 and BBa_K3365002 by Overlap PCR.
References
[1] Guzman LM, Belin D, Carson MJ, Beckwith J. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriol. 1995 Jul;177(14):4121-30. [2] Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim WA. Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression. Cell. 2013 Feb 28;152(5):1173-83. [3] Vigouroux A, Bikard D. CRISPR Tools To Control Gene Expression in Bacteria. Microbiol Mol Biol Rev. 2020 Apr 1;84(2):e00077-19.