Difference between revisions of "Part:BBa K3505019"

Latest revision as of 20:47, 27 October 2020

eGFP GB compatible with B3-B5

Level 0 eGFP

Fig.1:eGFP

Usage and Biology

Derived from jellyfish Aequeora victoria
A reporter gene fluorescent protein
Excitation at 488 nm
Emission at 515 nm

Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 BBa_K3505007and has overhangs compatible for GoldenBraid cloning. The CDS has position B3-B5.

Fig.2:The overhangs of this part in the GoldenBraid Grammar

Verification of cloning

Fig.3::(U=Uncut C=Cut) Restriction digestion of EGFP (C1-C4 ) with : PvuII, Expected bands : 2826 bp , Positive Result : C4

Experimental Use and Experience

This part is used in BBa_K3505029

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 17: / Line 17: @@
 ===Design Notes===
-The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 <bbpart>BBa_K3505007</bbpart>and has overhangs compatible for Golden Braid cloning.
+The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 <bbpart>BBa_K3505007</bbpart>and has overhangs compatible for GoldenBraid cloning.
 The CDS has position B3-B5.
-[[File:T--Thessaly--GB-AATG-GCTT.jpeg|700px|thumb|none|<i><b>Fig.2:</b>The overhangs of this part in the Golden Braid Grammar</i>]]
+[[File:T--Thessaly--GB-AATG-GCTT.jpeg|700px|thumb|none|<i><b>Fig.2:</b>The overhangs of this part in the GoldenBraid Grammar</i>]]
 ===Verification of cloning===
 [[File:T--Thessaly--egfp0.png|600px|thumb|none|<i><b>Fig.3:</b>:(U=Uncut C=Cut) Restriction digestion of EGFP (C1-C4 ) with : PvuII, Expected bands : 2826 bp , Positive Result : C4</i>]]