Difference between revisions of "Part:BBa K3544003:Design"
(→Design Notes) |
(→References) |
||
| (One intermediate revision by the same user not shown) | |||
| Line 11: | Line 11: | ||
===Source=== | ===Source=== | ||
| − | Botrytis | + | The original nucleotide sequence is from NCBI <i>Botrytis cinerea B05.10 chromosome 8, NC_037317.1</i>. The sequence is optimized according to the condon usage bias in <i>Saccharomyces cerevisiae</i> and synthesized from Sangon. |
===References=== | ===References=== | ||
| + | Siewers V, Kokkelink L, Smedsgaard J, et al. Identification of an abscisic acid gene cluster in the grey mold Botrytis cinerea[J]. Applied and Environmental Microbiology, 2006, 72(7): 4619-4626. | ||
Latest revision as of 14:00, 27 October 2020
Coding sequence of BcABA4, a member in biosynthetic gene cluster of ABA in B.cinerea
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 696
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 696
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 696
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 696
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
An enzyme involved in the synthesis of ABA in Botrytis cinerea. It can catalyze α-ionylideneacetic acid into abscisic acid.
Source
The original nucleotide sequence is from NCBI Botrytis cinerea B05.10 chromosome 8, NC_037317.1. The sequence is optimized according to the condon usage bias in Saccharomyces cerevisiae and synthesized from Sangon.
References
Siewers V, Kokkelink L, Smedsgaard J, et al. Identification of an abscisic acid gene cluster in the grey mold Botrytis cinerea[J]. Applied and Environmental Microbiology, 2006, 72(7): 4619-4626.