Difference between revisions of "Part:BBa K143012"
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Latest revision as of 16:23, 10 September 2012
Promoter veg: Constitutive Promoter for B. subtilis
Pveg is a constitutive promoter that constitutively expresses the P43 protein in B. subtilis. Pveg contains binding sites for the B. sutbilis major sigma factor, Sigma factor A 1. Pveg in B. subtilis utilises two binding sites to cause high expression of genes #2, however this Pveg is lacking the upstream site to give a medium level of gene expression. It has been noted that the sporulation master regulation factor spoOA interacts with Pveg though it is not known how #3. The context with which we used the promoter Pveg is as a Polymerase Per Second (PoPS) generator.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Part Characterisation
Please see the testing constructs BBa_K143079 and BBa_K143082 for characterisation data.
References
<biblio>
- 1 pmid=6181373
- 2 pmid=6820069
- 3 pmid=14651647
</biblio>