Difference between revisions of "Part:BBa K3544002:Design"

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===Design Notes===
 
===Design Notes===
−
An enzyme involved in the synthesis of ABA in Botrytis cinerea. It can catalyze Farnesyl diphosphate (FPP) into α-ionylideneethane.
+
An enzyme involved in the synthesis of ABA in <i>Botrytis cinerea</i>. It can catalyze Farnesyl diphosphate (FPP) into α-ionylideneethane.
  
 
===Source===
 
===Source===
  
−
Botrytis Cinerea
+
The original nucleotide sequence is from NCBI <i>Botrytis cinerea B05.10</i> chromosome 8, NC_037317.1. The sequence is optimized according to the condon usage bias in <i>Saccharomyces cerevisiae</i> and synthesized from Sangon
  
 
===References===
 
===References===
 +
Siewers V, Kokkelink L, Smedsgaard J, et al. Identification of an abscisic acid gene cluster in the grey mold Botrytis cinerea[J]. Applied and Environmental Microbiology, 2006, 72(7): 4619-4626.

Latest revision as of 13:49, 27 October 2020


Coding sequence of BcABA3, a member in biosynthetic gene cluster of ABA in B.cinerea


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 171
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 171
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 171
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 171
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

An enzyme involved in the synthesis of ABA in Botrytis cinerea. It can catalyze Farnesyl diphosphate (FPP) into α-ionylideneethane.

Source

The original nucleotide sequence is from NCBI Botrytis cinerea B05.10 chromosome 8, NC_037317.1. The sequence is optimized according to the condon usage bias in Saccharomyces cerevisiae and synthesized from Sangon

References

Siewers V, Kokkelink L, Smedsgaard J, et al. Identification of an abscisic acid gene cluster in the grey mold Botrytis cinerea[J]. Applied and Environmental Microbiology, 2006, 72(7): 4619-4626.