Difference between revisions of "Part:BBa K133114"

Latest revision as of 00:47, 30 October 2008

multiHP-Histop

Synthetic multiepitope, comprising the H. pylori urease B epitope, a vacuolating cytotoxin A (Vac) epitope and an adhesin A (HpaA) epitope. UreB epitope comprises AA 314-339 of Helicobacter pylori urease B, VacA AA 368-487 of vacuolating cytotoxin A of Helicobacter pylori and HpaA AA 134-146 of Helicobacter pylori. B-cell epitopes were designed using available 3D models or structures, which enabled more accurate prediction. Some of them were already shown to elicit antibody production, thus our combination broadens a repertoire of activated B-cells.

In this part, the multiepitope is fused with His-tag and contains a stop codon.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K133114 short</partinfo>
-Synthetic multiepitope, comprising the H. pylori urease B epitope, a vacuolating cytotoxin A (Vac) epitope and an adhesin A (HpaA) epitope. UreB epitope comprises AA 314-339 of ''Helicobacter pylori'' urease B, VacA AA 368-487 of vacuolating cytotoxin A of ''Helicobacter pylori'' and HpaA AA 134-146  of''Helicobacter pylori''. B-cell epitopes were designed using available 3D models or structures, which enabled more accurate prediction. Some of them were already shown to elicit antibody production, thus our combination broadens a repertoire of activated B-cells.
+Synthetic multiepitope, comprising the H. pylori urease B epitope, a vacuolating cytotoxin A (Vac) epitope and an adhesin A (HpaA) epitope. UreB epitope comprises AA 314-339 of ''Helicobacter pylori'' urease B, VacA AA 368-487 of vacuolating cytotoxin A of ''Helicobacter pylori'' and HpaA AA 134-146  of ''Helicobacter pylori''. B-cell epitopes were designed using available 3D models or structures, which enabled more accurate prediction. Some of them were already shown to elicit antibody production, thus our combination broadens a repertoire of activated B-cells.
+In this part, the multiepitope is fused with His-tag and contains a stop codon.