Difference between revisions of "Part:BBa K3657014:Design"

Latest revision as of 21:12, 27 October 2020

Antitermination protein N from Lambda Phage

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

The aminoacid sequence was obtained from the Uniprot database (P03045). The nucletide sequence was then generated and codon optimised for E. coli.

Source

Bacteriophage lambda

References

Parks AR, Court C, Lubkowska L, Jin DJ, Kashlev M, Court DL. Bacteriophage λ N protein inhibits transcription slippage by Escherichia coli RNA polymerase. Nucleic Acids Res. 2014 May;42(9):5823-9. doi: 10.1093/nar/gku203. Epub 2014 Apr 7. PMID: 24711367; PMCID: PMC4027172.

This part on Uniprot: https://www.uniprot.org/uniprot/P03045

@@ Line 8: / Line 8: @@
 ===Design Notes===
 {{#tag:html|
-The aminoacid sequence was obtained from the Uniprot database (<a href= "https://www.uniprot.org/uniprot/P03045">P03045</a>).
+The aminoacid sequence was obtained from the Uniprot database (P03045).
 The nucletide sequence was then generated and codon optimised for <i>E. coli</i>.
 }}
@@ Line 17: / Line 17: @@
 ===References===
-<br>Parks AR, Court C, Lubkowska L, Jin DJ, Kashlev M, Court DL. Bacteriophage λ N protein inhibits transcription slippage by Escherichia coli RNA polymerase. Nucleic Acids Res. 2014 May;42(9):5823-9. doi: 10.1093/nar/gku203. Epub 2014 Apr 7. PMID: 24711367; PMCID: PMC4027172.</br>
+<p>Parks AR, Court C, Lubkowska L, Jin DJ, Kashlev M, Court DL. Bacteriophage λ N protein inhibits transcription slippage by Escherichia coli RNA polymerase. Nucleic Acids Res. 2014 May;42(9):5823-9. doi: 10.1093/nar/gku203. Epub 2014 Apr 7. PMID: 24711367; PMCID: PMC4027172.</p>
+<p>This part on Uniprot: https://www.uniprot.org/uniprot/P03045</p>