Difference between revisions of "Part:BBa K084010:Experience"
 
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The part was tested using this [http://2008.igem.org/Team:Chiba/protocol/phenotype/timedelay protocol].
 
The part was tested using this [http://2008.igem.org/Team:Chiba/protocol/phenotype/timedelay protocol].
  
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One part of the senders cultures were mixed with 1 part of the receiver culture.
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One part of the senders cultures were mixed with 1 part of the receiver culture.(Mixed sender culture 1000μL with receiver culture 1000μL.)
  
 
[[image:Chiba_talks_JW1908_37_RS1_001.gif|frame|left|
 
[[image:Chiba_talks_JW1908_37_RS1_001.gif|frame|left|
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Senders strain:XL10Gold,Receiver strain:JW1908.Reaction temparature:30&deg;C.All measurements are averages from three replicate cultures with error bars representing standard deviations.Labeling:LasI,RhlI,RhlI(with LVA),CinI(with LVA),LuxI(with LVA) means fluorescence induced by AHLs synthesized by [https://parts.igem.org/Part:BBa_K084007 BBa_K084007],[https://parts.igem.org/Part:BBa_K084008 BBa_K084008],[https://parts.igem.org/Part:BBa_K084009 BBa_K084009],[https://parts.igem.org/Part:BBa_K0840010 BBa_K0840010],[https://parts.igem.org/Part:BBa_S03623 :BBa_S03623]respectively.]]<br clear=all>
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Senders strain:XL10Gold,Receiver strain:JW1908.Reaction temparature:30&deg;C.Labeling:LasI,RhlI,RhlI(with LVA),CinI(with LVA),LuxI(with LVA) means fluorescence induced by AHLs synthesized by [https://parts.igem.org/Part:BBa_K084007 BBa_K084007],[https://parts.igem.org/Part:BBa_K084008 BBa_K084008],[https://parts.igem.org/Part:BBa_K084009 BBa_K084009],[https://parts.igem.org/Part:BBa_K084010 BBa_K084010],[https://parts.igem.org/Part:BBa_S03623 :BBa_S03623]respectively.]]<br clear=all>
  
 
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Latest revision as of 09:05, 30 October 2008

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K084010

User Reviews

The iGEM Chiba team conducted tests on this part in the summer of 2008. The part was tested using this [http://2008.igem.org/Team:Chiba/protocol/phenotype/timedelay protocol].

One part of the senders cultures were mixed with 1 part of the receiver culture.(Mixed sender culture 1000μL with receiver culture 1000μL.)

Senders strain:XL10Gold,Receiver strain:JW1908.Reaction temparature:30°C.Labeling:LasI,RhlI,RhlI(with LVA),CinI(with LVA),LuxI(with LVA) means fluorescence induced by AHLs synthesized by BBa_K084007,BBa_K084008,BBa_K084009,BBa_K084010,:BBa_S03623respectively.

•••••

iGEMChiba 2008

The part was tested using this [http://2008.igem.org/Team:Chiba/protocol/phenotype/timedelay protocol].

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UNIQ3e6b1cf8260c2a71-partinfo-00000001-QINU UNIQ3e6b1cf8260c2a71-partinfo-00000002-QINU