Difference between revisions of "Part:BBa K3447111"
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<partinfo>BBa_K3447111 short</partinfo>
 
<partinfo>BBa_K3447111 short</partinfo>
  
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This part contains a agrAC gene, which constitutes the AIP sensing system. By connecting the sfGFP gene downstream, we were able to verify the expression intensity of this part.
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This part contains agrAC gene, which constitutes the AIP sensing system. By connecting the sfGFP gene downstream, we were able to verify the expression intensity of this part.
  
  
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To verify the construction of agrAC-P<sub>2</sub>-sfGFP, the digestion and agarose gel electrophoresis were performed by a standard protocol (Fig. 1).<br>
 
To verify the construction of agrAC-P<sub>2</sub>-sfGFP, the digestion and agarose gel electrophoresis were performed by a standard protocol (Fig. 1).<br>
  
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[[Image: Digestion and electrophoresis of agrAC.jpg|thumb|center|300px|<b>Figure 1. Digestion and electrophoresis of agrAC.</b>]]<br>
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[[Image: Digestion and electrophoresis of agrAC.jpg|thumb|300px|<b>Figure 1. Digestion and electrophoresis of agrAC.</b>]]<br>
  
 
==<b>Design</b>==
 
==<b>Design</b>==

Latest revision as of 08:47, 27 October 2020

AIP is induced and fluorescence is generated

This part contains agrAC gene, which constitutes the AIP sensing system. By connecting the sfGFP gene downstream, we were able to verify the expression intensity of this part.


Usage and Biology

AgrC protein is the reporter for AIP. Upon binding to AIP, AgrC dimerizes and phosphorylates each other to activate AgrC proteins. AgrC can phosphorylate AgrA protein. The phosphorylated AgrA protein dimerizes and binds to the P2 promoter of the agrBDCA operon to form a positive feedback loop.

Characterization

To verify the construction of agrAC-P2-sfGFP, the digestion and agarose gel electrophoresis were performed by a standard protocol (Fig. 1).

Figure 1. Digestion and electrophoresis of agrAC.

Design

Design Notes

We added some synonymous mutations to avoid part rules.


Source

We found this sequence data in GenBank.

References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 26
    Illegal NheI site found at 49
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2803
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1