Difference between revisions of "Part:BBa K3329066"

Latest revision as of 09:17, 27 October 2020

superfolder GFP optimized V4

The superfolder GFP protein is connected with scl2. The green color indicates whether the scl2 is bound to cellulose by seeing whether that filter paper turned green.

It's noteworthy that sfGFP would be expressed in both P. Pastoris and E. coli, respectively. Therefore, codon optimization must be applied in order to avoid homologous recombination and fit the chassis organism. As a result, we synthesized 4 versions of genes coding the same sfGFP sequence based on the 2 versions of codon optimization for P. Pastoris and 2 versions for the codon optimization of E. coli. Names for those 4 versions of CDS are documented from sfGFP optimized V1 to CBM optimized V4, in which the sfGFP optimized V1 and V2 are for P. Pastoris, and sfGFP optimized V3-4 are for E. coli.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 83
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 562
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3329066 short</partinfo>
-The superfolder GFP protein is connected with scl2. The green color indicates whether thescl2 is bound to cellulose by seeing whether that filter paper turned green.
+The superfolder GFP protein is connected with scl2. The green color indicates whether the scl2 is bound to cellulose by seeing whether that filter paper turned green.
+It's noteworthy that sfGFP would be expressed in both P. Pastoris and E. coli, respectively. Therefore, codon optimization must be applied in order to avoid homologous recombination and fit the chassis organism. As a result, we synthesized 4 versions of genes coding the same sfGFP sequence based on the 2 versions of codon optimization for P. Pastoris and 2 versions for the codon optimization of E. coli. Names for those 4 versions of CDS are documented from sfGFP optimized V1 to CBM optimized V4, in which the sfGFP optimized V1 and V2 are for P. Pastoris, and sfGFP optimized V3-4 are for E. coli.
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