Difference between revisions of "Part:BBa K3406008:Design"
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===Design Notes=== | ===Design Notes=== | ||
We use Recombinase Polymerase Amplification to amplify a short sequence of H1N1 genome. To maximize amplification success, the primer lengths should be ~25–35 nt, and the total amplicon size should be 80–140 bp. Primers are typically designed with melting temperatures between 54 and 67 °C. In addition, because we need to do transcription experiments downstream, a T7RNA polymerase promoter was appended to the 5ʹend of the forward primers to allow T7 transcription. | We use Recombinase Polymerase Amplification to amplify a short sequence of H1N1 genome. To maximize amplification success, the primer lengths should be ~25–35 nt, and the total amplicon size should be 80–140 bp. Primers are typically designed with melting temperatures between 54 and 67 °C. In addition, because we need to do transcription experiments downstream, a T7RNA polymerase promoter was appended to the 5ʹend of the forward primers to allow T7 transcription. | ||
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| + | ===Source=== | ||
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| + | ===References=== | ||
Latest revision as of 07:14, 27 October 2020
RPA forward primer for H1N1
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 31
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 31
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 31
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We use Recombinase Polymerase Amplification to amplify a short sequence of H1N1 genome. To maximize amplification success, the primer lengths should be ~25–35 nt, and the total amplicon size should be 80–140 bp. Primers are typically designed with melting temperatures between 54 and 67 °C. In addition, because we need to do transcription experiments downstream, a T7RNA polymerase promoter was appended to the 5ʹend of the forward primers to allow T7 transcription.