Difference between revisions of "Part:BBa K3645016"

 
 
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<partinfo>BBa_K3645016 short</partinfo>
 
<partinfo>BBa_K3645016 short</partinfo>
  
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Template of sgRNA. Use Golden Gate (Bsa&#8544;) to replace the template sequence with a ~20bp guide sequence. Transcription product is a single strain sgRNA for dCas9.  
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Template of sgRNA. Use Golden Gate (Bsa&#8544;) to replace the template sequence with a ~20bp guide sequence. Add 'tagc' on the 5'-terminal of your guide sequence and 'aaac' on the reverse 5'-terminal.
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5'-tagc-20bp-****-3'<br>
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3'-****-20bp-caaa-5'
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Transcription product is a single strain sgRNA for dCas9.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 07:17, 27 October 2020


sgRNA scaffold

Template of sgRNA. Use Golden Gate (BsaⅠ) to replace the template sequence with a ~20bp guide sequence. Add 'tagc' on the 5'-terminal of your guide sequence and 'aaac' on the reverse 5'-terminal.

5'-tagc-20bp-****-3'
3'-****-20bp-caaa-5'

Transcription product is a single strain sgRNA for dCas9.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 135
    Illegal BsaI.rc site found at 37