Difference between revisions of "Part:BBa K3406008:Design"

 
 
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<partinfo>BBa_K3406008 short</partinfo>
  
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<partinfo>BBa_K3406008 SequenceAndFeatures</partinfo>
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===Design Notes===
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We use Recombinase Polymerase Amplification to amplify a short sequence of H1N1 genome. To maximize amplification success, the primer lengths should be ~25–35 nt, and the total amplicon size should be 80–140 bp. Primers are typically designed with melting temperatures between 54 and 67 °C. In addition, because we need to do transcription experiments downstream, a T7RNA polymerase promoter was appended to the 5ʹend of the forward primers to allow T7 transcription.
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===Source===
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===References===

Latest revision as of 07:14, 27 October 2020

RPA forward primer for H1N1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 31
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 31
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 31
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use Recombinase Polymerase Amplification to amplify a short sequence of H1N1 genome. To maximize amplification success, the primer lengths should be ~25–35 nt, and the total amplicon size should be 80–140 bp. Primers are typically designed with melting temperatures between 54 and 67 °C. In addition, because we need to do transcription experiments downstream, a T7RNA polymerase promoter was appended to the 5ʹend of the forward primers to allow T7 transcription.

Source

References