Difference between revisions of "Part:BBa K3526004"
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K3526004 short</partinfo>
 
<partinfo>BBa_K3526004 short</partinfo>
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<partinfo>BBa_K3526004 SequenceAndFeatures</partinfo>
  
 
A transcription factor, atzR, which can sense the presence of cyanuric acid.
 
A transcription factor, atzR, which can sense the presence of cyanuric acid.
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===Contribution===
 
===Contribution===
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BBa_K3526004 is a composite part contains a special transcription factor AtzR. This part should be used with the part BBa_K3526005. Under the presence of cyanuric acid (CYA), the AtzR can bind CYA and regulate the promoter PatzD. As a result, the GFP is expressed and emit green fluorescence.
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BBa_K3526004 is a composite part that contains a special transcription factor AtzR. This part should be used with the part BBa_K3526005. Under the presence of cyanuric acid (CYA), the AtzR can bind CYA and regulate the promoter PatzD. As a result, the GFP is expressed and emit green fluorescence.
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[[File:T--Shanghai city-BBa K3526004 fig 0.png|500px|thumb|center|Figure 1]]
  
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[[File: File.png|500px|thumb|center|figure 1]]
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[[File:T--Shanghai city-BBa_K3526004 fig 00.png|500px|thumb|center|Figure 2]]
  
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[[File: File.png|500px|thumb|center|figure 2]]
 
 
===Engineering Success===
 
===Engineering Success===
 
To test the function of part BBa_K3526004, we co-transform the part BBa_K3526004 and part BBa_K3526005 into Pseudomonas putida KT2440. After reading related articles, we choose 0μM, 50μM, and 100μM as the detection concentrations of CYA. After preliminary analysis, we obtained Figure 3.
 
To test the function of part BBa_K3526004, we co-transform the part BBa_K3526004 and part BBa_K3526005 into Pseudomonas putida KT2440. After reading related articles, we choose 0μM, 50μM, and 100μM as the detection concentrations of CYA. After preliminary analysis, we obtained Figure 3.
  
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[[File: File.png|500px|thumb|center|figure 3]]
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[[File:T--Shanghai city-BBa_K3526004 fig 1.png|500px|thumb|center|Figure 3]]
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                                                              Figure 3
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The results of the experiment show that with the presence of part BBa_K3526004 and BBa_K3526005, pseudomonas putida KT2440 can detect cyanuric acid.
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The results of the experiment show that with the presence of part BBa_K3526004 and BBa_K3526005, Pseudomonas putida KT2440 can detect cyanuric acid.

Latest revision as of 08:34, 27 October 2020

tac pro-atzR-T4 ter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1026
    Illegal AgeI site found at 864
  • 1000
    COMPATIBLE WITH RFC[1000]

A transcription factor, atzR, which can sense the presence of cyanuric acid.


Contribution

BBa_K3526004 is a composite part that contains a special transcription factor AtzR. This part should be used with the part BBa_K3526005. Under the presence of cyanuric acid (CYA), the AtzR can bind CYA and regulate the promoter PatzD. As a result, the GFP is expressed and emit green fluorescence.

Figure 1
Figure 2

Engineering Success

To test the function of part BBa_K3526004, we co-transform the part BBa_K3526004 and part BBa_K3526005 into Pseudomonas putida KT2440. After reading related articles, we choose 0μM, 50μM, and 100μM as the detection concentrations of CYA. After preliminary analysis, we obtained Figure 3.

Figure 3

The results of the experiment show that with the presence of part BBa_K3526004 and BBa_K3526005, Pseudomonas putida KT2440 can detect cyanuric acid.