Difference between revisions of "Part:BBa K3484003"
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<partinfo>BBa_K3484003 short</partinfo> | <partinfo>BBa_K3484003 short</partinfo> | ||
| − | + | This part is the transcriptional unit for <partinfo>BBa_K3484002</partinfo>. This part expresses constitutively an intein-mediated T3 biosensor with enhanced GFP (eGFP) as a reporter (IMT3_eGFP). This part was an intermediate sensor to later on develop the IMT3_sfGFP (<partinfo>BBa_K3484000</partinfo>). Visit its page to see the characterization and final design. | |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 20:29, 27 October 2020
Intein-mediated T3(THRB) → eGFP (transcriptional unit)
This part is the transcriptional unit for BBa_K3484002. This part expresses constitutively an intein-mediated T3 biosensor with enhanced GFP (eGFP) as a reporter (IMT3_eGFP). This part was an intermediate sensor to later on develop the IMT3_sfGFP (BBa_K3484000). Visit its page to see the characterization and final design.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 235
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 725
Illegal NgoMIV site found at 2228 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1640