Difference between revisions of "Part:BBa K3593006"
| (One intermediate revision by one other user not shown) | |||
| Line 4: | Line 4: | ||
The capture strand composes the control line of our test strip, which is complementary to the reporter probe on Au nanoparticle. This allows the capture of Au particle at any time with a toxin or not, to verify the function of lateral flow assay. | The capture strand composes the control line of our test strip, which is complementary to the reporter probe on Au nanoparticle. This allows the capture of Au particle at any time with a toxin or not, to verify the function of lateral flow assay. | ||
| − | |||
=Sequence and features= | =Sequence and features= | ||
<partinfo>BBa_K3593006 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3593006 SequenceAndFeatures</partinfo> | ||
=Characterisation of this part= | =Characterisation of this part= | ||
==Design== | ==Design== | ||
| − | + | Besides aptamers, we have some short single stranded DNA known as 'probes', which binds to the reporter probe on Au nanoparticle. BBa_K3593006 is one of those so-called 'probes' in our design, in which it reports a red color anyway on the control line, to verify the function of lateral flow construction.[1] | |
=References= | =References= | ||
| + | 1.Jauset-Rubio M, Svobodová M, Mairal T, et al. Aptamer Lateral Flow Assays for Ultrasensitive Detection of β-Conglutin Combining Recombinase Polymerase Amplification and Tailed Primers. Anal Chem. 2016;88(21):10701-10709. doi:10.1021/acs.analchem.6b03256<br> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 03:44, 28 October 2020
ssDNA, capture probe of control line for RPA method
The capture strand composes the control line of our test strip, which is complementary to the reporter probe on Au nanoparticle. This allows the capture of Au particle at any time with a toxin or not, to verify the function of lateral flow assay.
Sequence and features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterisation of this part
Design
Besides aptamers, we have some short single stranded DNA known as 'probes', which binds to the reporter probe on Au nanoparticle. BBa_K3593006 is one of those so-called 'probes' in our design, in which it reports a red color anyway on the control line, to verify the function of lateral flow construction.[1]
References
1.Jauset-Rubio M, Svobodová M, Mairal T, et al. Aptamer Lateral Flow Assays for Ultrasensitive Detection of β-Conglutin Combining Recombinase Polymerase Amplification and Tailed Primers. Anal Chem. 2016;88(21):10701-10709. doi:10.1021/acs.analchem.6b03256