Difference between revisions of "Part:BBa K3657052"
 
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<partinfo>BBa_K3657052 short</partinfo>
 
<partinfo>BBa_K3657052 short</partinfo>
 
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This part consists of a sequence that forms an RNA&middot;DNA-DNA triple helix (DNA part: <a href="https://parts.igem.org/Part:BBa_K3657023">BBa_K3657023</a>) and a sequence that forms a BoxB hairpin (<a href="https://parts.igem.org/Part:BBa_K3657015">BBa_K3657015</a>).  
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<p>This part consists of a sequence that forms an RNA&middot;DNA-DNA triple helix (DNA part: <a href="https://parts.igem.org/Part:BBa_K3657023">BBa_K3657023</a>) and a sequence that forms a BoxB hairpin (<a href="https://parts.igem.org/Part:BBa_K3657015">BBa_K3657015</a>). </p>
  
 
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<p>The BoxB hairpin binds to the lambdaN protein (<a href="https://parts.igem.org/Part:BBa_K3657014">BBa_K3657014</a>). The triple helix forming part binds the DNA sequence specifically. In that way this part should theoretically attract a protein of interest fused to a lambdaN protein to a specific region on DNA. Due to the COVID19 pandemic we were not able to get any empirical evidence that this part functions as described above. More information available here: <a href="https://2020.igem.org/Team:Heidelberg/Triple_Helix">iGEM Heidelberg 2020.</a></p>
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<img style="width:75%" src="https://2020.igem.org/wiki/images/0/0c/T--Heidelberg--BBa_K3657052_structure.jpeg">
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<p><b>Figure 1: Secondary structure of BBa_K3657052</b> <br>
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MS2 hairpin forms a stable structure. The triple helix forming sequence doesn't form any stable structure and does not interfere with the BoxB hairpin. The structure was generated by the Geneious software.</p>
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3657052 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3657052 SequenceAndFeatures</partinfo>

Latest revision as of 00:29, 27 October 2020


RNA Part of an RNA·DNA-DNA triple helix + BoxB

This part consists of a sequence that forms an RNA·DNA-DNA triple helix (DNA part: BBa_K3657023) and a sequence that forms a BoxB hairpin (BBa_K3657015).

The BoxB hairpin binds to the lambdaN protein (BBa_K3657014). The triple helix forming part binds the DNA sequence specifically. In that way this part should theoretically attract a protein of interest fused to a lambdaN protein to a specific region on DNA. Due to the COVID19 pandemic we were not able to get any empirical evidence that this part functions as described above. More information available here: iGEM Heidelberg 2020.


Figure 1: Secondary structure of BBa_K3657052
MS2 hairpin forms a stable structure. The triple helix forming sequence doesn't form any stable structure and does not interfere with the BoxB hairpin. The structure was generated by the Geneious software.

Sequence and Features
Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]