Difference between revisions of "Part:BBa K3331004"

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<partinfo>BBa_K3331004 short</partinfo>
 
<partinfo>BBa_K3331004 short</partinfo>
  
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This part catalyzes the interconversion of alpha-D-glucose 1-phosphate and D-glucose 6-phosphate.  
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pgmA [Pseudomonas aeruginosa PAO1 (strain: PAO1)]
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<div>Encodes Phosphomannomutase, a highly reversible phosphoryltransferase that catalyzes the interconversion of α-D-mannose 1-phosphate and D-mannose 6-phosphate.</div>
 
This part comes from the genome of Pseudomonas aeruginosa. We optimized the sequence with the codon preferred by Bacillus subtilis. In this way, it can be more efficiently expressed in Bacillus subtilis.  
 
This part comes from the genome of Pseudomonas aeruginosa. We optimized the sequence with the codon preferred by Bacillus subtilis. In this way, it can be more efficiently expressed in Bacillus subtilis.  
  
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===Characterization===
 
===Characterization===
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We cloned and confirmed the target fragment by PCR. The length of our fragment is 1521bp. The following results showed the successful result.
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We cloned and confirmed the target fragment by PCR. The length of our fragment is 1392bp. The following results showed the successful result.
 
<div>[[File:T--XJTU-China--P.pgel.png |700px|thumb|center|<b>Figure 1:</b>PCR confirmation of the target fragment.]]</div>
 
<div>[[File:T--XJTU-China--P.pgel.png |700px|thumb|center|<b>Figure 1:</b>PCR confirmation of the target fragment.]]</div>

Latest revision as of 10:56, 27 October 2020


P.A. pgmA

pgmA [Pseudomonas aeruginosa PAO1 (strain: PAO1)]

Encodes Phosphomannomutase, a highly reversible phosphoryltransferase that catalyzes the interconversion of α-D-mannose 1-phosphate and D-mannose 6-phosphate.

This part comes from the genome of Pseudomonas aeruginosa. We optimized the sequence with the codon preferred by Bacillus subtilis. In this way, it can be more efficiently expressed in Bacillus subtilis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 672
    Illegal BglII site found at 705
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 27
    Illegal AgeI site found at 261
    Illegal AgeI site found at 900
    Illegal AgeI site found at 1278
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

We cloned and confirmed the target fragment by PCR. The length of our fragment is 1392bp. The following results showed the successful result.

Figure 1:PCR confirmation of the target fragment.