Difference between revisions of "Part:BBa K3331003"
 
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<partinfo>BBa_K3331003 short</partinfo>
 
<partinfo>BBa_K3331003 short</partinfo>
  
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This part catalyzes the interconversion of alpha-D-glucose 1-phosphate and D-glucose 6-phosphate.
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pgmA [Bacillus subtilis (strain 168)]
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This part comes from the genome of  Bacillus subtilis.
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<div>Encodes 2,3-bisphosphoglycerate-independent phosphoglycerate mutase. This enzyme catalyzes the reversible reaction between 2-phosphoglycerate and 3-phosphoglycerate</div>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
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===Characterization===
 
===Characterization===
 
We cloned and confirmed the target fragment by PCR. The length of our fragment is 1875bp. The following results showed the successful result.
 
We cloned and confirmed the target fragment by PCR. The length of our fragment is 1875bp. The following results showed the successful result.
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<div>[[File:T--XJTU-China--Bggel.png |700px|thumb|center|<b>Figure 1:</b>PCR confirmation of the target fragment.]]</div>
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<div>[[File:T--XJTU-China--B.pgel.png |700px|thumb|center|<b>Figure 1:</b>PCR confirmation of the target fragment.]]</div>

Latest revision as of 10:52, 27 October 2020


B.S. pgmA

pgmA [Bacillus subtilis (strain 168)]

Encodes 2,3-bisphosphoglycerate-independent phosphoglycerate mutase. This enzyme catalyzes the reversible reaction between 2-phosphoglycerate and 3-phosphoglycerate

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

We cloned and confirmed the target fragment by PCR. The length of our fragment is 1875bp. The following results showed the successful result.

Figure 1:PCR confirmation of the target fragment.