Difference between revisions of "Part:BBa K3331002"
 
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<partinfo>BBa_K3331002 short</partinfo>
 
<partinfo>BBa_K3331002 short</partinfo>
  
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This part catalyzes the interconversion of alpha-D-glucose 1-phosphate and D-glucose 6-phosphate.
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pgmA [Escherichia coli (strain K12)]
  
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This part comes from the genome of E.coli. We optimized the sequence with the codon preferred by Bacillus subtilis. In this way, it can be more efficiently expressed in Bacillus subtilis.
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Encodes 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase that catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate.
 +
 
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This part can catalyzes the interconversion of alpha-D-glucose 1-phosphate and D-glucose 6-phosphate.
 +
We optimized the sequence with the codon preferred by Bacillus subtilis. In this way, it can be more efficiently expressed in Bacillus subtilis.
  
  
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K3331002 parameters</partinfo>
 
<partinfo>BBa_K3331002 parameters</partinfo>
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===Characterization===
 
===Characterization===
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We cloned and confirmed the target fragment by PCR. The length of our fragment is 981bp. The following results showed the successful result.
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We cloned and confirmed the target fragment by PCR. The length of our fragment is 1770bp. The following results showed the successful result.
 
<div>[[File:T--XJTU-China--E.pgel.png |700px|thumb|center|<b>Figure 1:</b>PCR confirmation of the target fragment.]]</div>
 
<div>[[File:T--XJTU-China--E.pgel.png |700px|thumb|center|<b>Figure 1:</b>PCR confirmation of the target fragment.]]</div>

Latest revision as of 10:47, 27 October 2020


E.coli pgmA

pgmA [Escherichia coli (strain K12)]

Encodes 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase that catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate.

This part can catalyzes the interconversion of alpha-D-glucose 1-phosphate and D-glucose 6-phosphate. We optimized the sequence with the codon preferred by Bacillus subtilis. In this way, it can be more efficiently expressed in Bacillus subtilis.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Characterization

We cloned and confirmed the target fragment by PCR. The length of our fragment is 1770bp. The following results showed the successful result.

Figure 1:PCR confirmation of the target fragment.