Difference between revisions of "Part:BBa K084012:Experience"
 
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The iGEM Chiba team conducted tests on this part in the summer of 2008.
 
The iGEM Chiba team conducted tests on this part in the summer of 2008.
 
The part was tested using this [http://2008.igem.org/Team:Chiba/protocol/phenotype/timedelay protocol].
 
The part was tested using this [http://2008.igem.org/Team:Chiba/protocol/phenotype/timedelay protocol].
 +
 +
One part of the senders cultures were mixed with 1 part of the receiver culture.
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(Mixed sender culture 500μL with receiver culture 500μL.)
  
 
[[image:senders_crosstalk_chiba_01.gif|frame|left|
 
[[image:senders_crosstalk_chiba_01.gif|frame|left|
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senders crosstalk test.senders strain XL10Gold,Receiver strain JW1908.Reaction temparature was 30°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.Labeling:LuxI,RhlI,LasI means fluorescence induced by AHLs synthesized by [https://parts.igem.org/Part:BBa_K0840012 BBa_K0840012],[https://parts.igem.org/Part:BBa_K084008 BBa_K084008],[https://parts.igem.org/Part:BBa_K084007 BBa_K084007] respectively.]][[image:Chiba_talks_XL10Gold_37_RS1_0923_01.gif|frame|left|senders crosstalk test.senders strain XL10Gold,Receiver strain JW1908.Reaction temparature was 37°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.Labeling:LuxI,RhlI,LasI means fluorescence induced by AHLs synthesized by [https://parts.igem.org/Part:BBa_K0840012 BBa_K0840012],[https://parts.igem.org/Part:BBa_K084008 BBa_K084008],[https://parts.igem.org/Part:BBa_K084007 BBa_K084007] respectively.]]
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senders crosstalk test.senders strain XL10Gold,Receiver strain JW1908.Reaction temparature was 30°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.Labeling:LuxI,RhlI,LasI means fluorescence induced by AHLs synthesized by [https://parts.igem.org/Part:BBa_K084012 BBa_K084012],[https://parts.igem.org/Part:BBa_K084008 BBa_K084008],[https://parts.igem.org/Part:BBa_K084007 BBa_K084007] respectively.]][[image:Chiba_talks_XL10Gold_37_RS1_0923_01.gif|frame|left|senders crosstalk test.senders strain XL10Gold,Receiver strain JW1908.Reaction temparature was 37°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.Labeling:LuxI,RhlI,LasI means fluorescence induced by AHLs synthesized by [https://parts.igem.org/Part:BBa_K084012 BBa_K084012],[https://parts.igem.org/Part:BBa_K084008 BBa_K084008],[https://parts.igem.org/Part:BBa_K084007 BBa_K084007] respectively.]]
  
 
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{|width='80%' style='border:1px solid gray'

Latest revision as of 09:15, 30 October 2008

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K084012

User Reviews

The iGEM Chiba team conducted tests on this part in the summer of 2008. The part was tested using this [http://2008.igem.org/Team:Chiba/protocol/phenotype/timedelay protocol].

One part of the senders cultures were mixed with 1 part of the receiver culture. (Mixed sender culture 500μL with receiver culture 500μL.)

senders crosstalk test.senders strain XL10Gold,Receiver strain JW1908.Reaction temparature was 30°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.Labeling:LuxI,RhlI,LasI means fluorescence induced by AHLs synthesized by BBa_K084012,BBa_K084008,BBa_K084007 respectively.
senders crosstalk test.senders strain XL10Gold,Receiver strain JW1908.Reaction temparature was 37°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.Labeling:LuxI,RhlI,LasI means fluorescence induced by AHLs synthesized by BBa_K084012,BBa_K084008,BBa_K084007 respectively.
•••••

iGEMChiba 2008

The part was tested using this [http://2008.igem.org/Team:Chiba/protocol/phenotype/timedelay protocol].

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UNIQebfe9601850b8252-partinfo-00000001-QINU UNIQebfe9601850b8252-partinfo-00000002-QINU