Difference between revisions of "Part:BBa K084007:Experience"
 
(7 intermediate revisions by 2 users not shown)
Line 9: Line 9:
 
The part was tested using this [http://2008.igem.org/Team:Chiba/protocol/phenotype/timedelay protocol].
 
The part was tested using this [http://2008.igem.org/Team:Chiba/protocol/phenotype/timedelay protocol].
  
−
[[image:senders_crosstalk_chiba_01.gif|thumb|left|
+
One part of the senders cultures were mixed with 1 part of the receiver culture.
−
senders crosstalk test.senders strain XL10Gold,Receiver strain JW1908.Reaction temparature was 30°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.Labeling:LuxI,RhlI,LasI means fluorescence induced by AHLs synthesized by [https://parts.igem.org/Part:BBa_K0840012 BBa_K0840012],[https://parts.igem.org/Part:BBa_K084008 BBa_K084008],[https://parts.igem.org/Part:BBa_K084007 BBa_K084007] respectively.]][[image:Chiba_talks_XL10Gold_37_RS1_0923_01.gif|thumb|left|senders crosstalk test.senders strain XL10Gold,Receiver strain JW1908.Reaction temparature was 37°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.Labeling:LuxI,RhlI,LasI means fluorescence induced by AHLs synthesized by [https://parts.igem.org/Part:BBa_K0840012 BBa_K0840012],[https://parts.igem.org/Part:BBa_K084008 BBa_K084008],[https://parts.igem.org/Part:BBa_K084007 BBa_K084007] respectively.]]
+
(Mixed sender culture 500μL with receiver culture 500μL.)
 +
[[image:senders_crosstalk_chiba_01.gif|frame|left|Senders strain:XL10Gold,Receiver strain:JW1908.Reaction temparature:30°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.Labeling:LuxI,RhlI,LasI means fluorescence induced by AHLs synthesized by [https://parts.igem.org/Part:BBa_K0840012 BBa_K0840012],[https://parts.igem.org/Part:BBa_K084008 BBa_K084008],[https://parts.igem.org/Part:BBa_K084007 BBa_K084007] respectively.]][[image:Chiba_talks_XL10Gold_37_RS1_0923_01.gif|frame|left|Senders strain:XL10Gold,Receiver strain:JW1908.Reaction temparature:37°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.Labeling:LuxI,RhlI,LasI means fluorescence induced by AHLs synthesized by [https://parts.igem.org/Part:BBa_K084012 BBa_K084012],[https://parts.igem.org/Part:BBa_K084008 BBa_K084008],[https://parts.igem.org/Part:BBa_K084007 BBa_K084007] respectively.]]
 +
<br clear=all>
  
 
{|width='80%' style='border:1px solid gray'
 
{|width='80%' style='border:1px solid gray'

Latest revision as of 09:14, 30 October 2008

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K084007

User Reviews

The iGEM Chiba team conducted tests on this part in the summer of 2008. The part was tested using this [http://2008.igem.org/Team:Chiba/protocol/phenotype/timedelay protocol].

One part of the senders cultures were mixed with 1 part of the receiver culture. (Mixed sender culture 500μL with receiver culture 500μL.)

Senders strain:XL10Gold,Receiver strain:JW1908.Reaction temparature:30°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.Labeling:LuxI,RhlI,LasI means fluorescence induced by AHLs synthesized by BBa_K0840012,BBa_K084008,BBa_K084007 respectively.
Senders strain:XL10Gold,Receiver strain:JW1908.Reaction temparature:37°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.Labeling:LuxI,RhlI,LasI means fluorescence induced by AHLs synthesized by BBa_K084012,BBa_K084008,BBa_K084007 respectively.


•••••

iGEMChiba 2008

The part was tested using this [http://2008.igem.org/Team:Chiba/protocol/phenotype/timedelay protocol].

;

UNIQ7096cddbb5a60a6e-partinfo-00000001-QINU UNIQ7096cddbb5a60a6e-partinfo-00000002-QINU