Difference between revisions of "Part:BBa K165078:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K165078=== | ===Applications of BBa_K165078=== | ||
| + | In a strain into which we transformed the LexA activator and LexA activator reporter, we found this part to be functional. After growing in 2% galactose/2%raffinose, under the confocal fluorescence microscope we saw mCherry expression in the nucleus (on the activating transcription factor), and its effect of inducing YFP expression in the cytosol from the activator reporter. See below: | ||
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| + | [[Image:Fluorotest.png|center]] | ||
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| + | The mCherry on the activator is red in the nucleus, and the YFP is green in cytosol. | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 06:16, 30 October 2008
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K165078
In a strain into which we transformed the LexA activator and LexA activator reporter, we found this part to be functional. After growing in 2% galactose/2%raffinose, under the confocal fluorescence microscope we saw mCherry expression in the nucleus (on the activating transcription factor), and its effect of inducing YFP expression in the cytosol from the activator reporter. See below:
The mCherry on the activator is red in the nucleus, and the YFP is green in cytosol.
User Reviews
UNIQcfafb13dc7395f94-partinfo-00000000-QINU UNIQcfafb13dc7395f94-partinfo-00000001-QINU