Difference between revisions of "Part:BBa K3396003"
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This is the fusion protein of GFP nanobody with FKBP on its C terminus. | This is the fusion protein of GFP nanobody with FKBP on its C terminus. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | FKBP is a kind of protein which can bind with FRB induced by rapamycin. GFPnano is the nanobody of GFP, a green fluorescent protein, which can be observed easily. This part could target GFP and bind TRIM21-FRB thought the FRB-FKBP interaction and lead to the degradation induced by ubiquitin-proteasome. | |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K3396003 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3396003 SequenceAndFeatures</partinfo> | ||
Latest revision as of 17:44, 26 October 2020
GFPnano-FKBP
This is the fusion protein of GFP nanobody with FKBP on its C terminus.
Usage and Biology
FKBP is a kind of protein which can bind with FRB induced by rapamycin. GFPnano is the nanobody of GFP, a green fluorescent protein, which can be observed easily. This part could target GFP and bind TRIM21-FRB thought the FRB-FKBP interaction and lead to the degradation induced by ubiquitin-proteasome.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 35