Difference between revisions of "Part:BBa K3505017"
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<partinfo>BBa_K3505017 short</partinfo> | <partinfo>BBa_K3505017 short</partinfo> | ||
− | + | <partinfo>BBa_B0015 parameters</partinfo> | |
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<p style="text-align: center;"> | <p style="text-align: center;"> | ||
− | [[Image:T--Thessaly--GB-GCTT-CGCT.jpeg |jpeg|900px|thumb|none|<i><b>Figure 1.</b>The overhangs of this part in the | + | [[Image:T--Thessaly--GB-GCTT-CGCT.jpeg |jpeg|900px|thumb|none|<i><b>Figure 1.</b>The overhangs of this part in the GoldenBraid Grammar.</i>]] |
[[Image:T--Thessaly-double terminator.png|900px|thumb|none|<I><b>Figure 2.</b> The level 0 module : pupd2- double terminator. (illustration from SnapGene)</i>]] | [[Image:T--Thessaly-double terminator.png|900px|thumb|none|<I><b>Figure 2.</b> The level 0 module : pupd2- double terminator. (illustration from SnapGene)</i>]] | ||
===Verification of Cloning=== | ===Verification of Cloning=== | ||
− | [[File:T--Thessaly-terminator-digestion.png|700px|thumb|none|<i><b>Fig.3:</b>(U=Uncut C=Cut) Restriction digestion of Terminator (C3 )with : | + | [[File:T--Thessaly--terminator-teliko-digestion.png|700px|thumb|none|<i><b>Fig.3:</b>(U=Uncut C=Cut) Restriction digestion of Terminator (C3 )with : |
EcoRI & PstI ( C3 ), Expected bands : 2029 + 209 bp , Positive result : C3</i>]] | EcoRI & PstI ( C3 ), Expected bands : 2029 + 209 bp , Positive result : C3</i>]] | ||
+ | |||
+ | ===Source=== | ||
+ | Synthesized by IDT. | ||
+ | |||
+ | ===Sequence and Features=== | ||
+ | <partinfo>BBa_K3505017 SequenceAndFeatures</partinfo> |
Latest revision as of 20:31, 27 October 2020
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Double Terminator GB compatible with B6-C1
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Design Notes
The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is presents in pUPD2 and has overhangs compatible for Golden Braid cloning. The CDS has position B6-C1.
Verification of Cloning
Source
Synthesized by IDT.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]