Difference between revisions of "Part:BBa K3424001:Design"
 
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=== Plasmid Design Notes===
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__NOTOC__
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<partinfo>BBa_K3424001 short</partinfo>
  
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Make sure to check the reference source paper and type in the sequence of the terminator to see how the authors designed it. If you want to design another gene after this terminator in your plasmid, you should have around 19 intermediate bases before inserting a promoter for your other gene.
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<partinfo>BBa_K3424001 SequenceAndFeatures</partinfo>
  
  
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===Reference Source===
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===Design Notes===
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N/A
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===Source===
  
 
Meyer, A.J., Segall-Shapiro, T.H., Glassey, E. et al. Escherichia coli “Marionette” strains with 12 highly optimized small-molecule sensors. Nat Chem Biol 15, 196–204 (2019). https://doi.org/10.1038/s41589-018-0168-3
 
Meyer, A.J., Segall-Shapiro, T.H., Glassey, E. et al. Escherichia coli “Marionette” strains with 12 highly optimized small-molecule sensors. Nat Chem Biol 15, 196–204 (2019). https://doi.org/10.1038/s41589-018-0168-3
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===References===

Latest revision as of 14:42, 27 October 2020

Terminator_TetR_L3S3P21


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 46


Design Notes

N/A


Source

Meyer, A.J., Segall-Shapiro, T.H., Glassey, E. et al. Escherichia coli “Marionette” strains with 12 highly optimized small-molecule sensors. Nat Chem Biol 15, 196–204 (2019). https://doi.org/10.1038/s41589-018-0168-3


References