Difference between revisions of "Part:BBa K3467003:Experience"
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As the result shows: | As the result shows: | ||
| − | + | [[File:T--CAU_China--CHS_dsRNA_analysis.jpg|1000px|thumb|center|'''Fig. 1''' CHS dsRNA injection experiment]] | |
Comparing with the WT and control group (by injecting DEPC water) locust survival rate, we used LT50 to measure dsRNA effect. From the diagram above, CHS dsRNA injection has a great effect to locust which CHS experiment group LT50 is approximately 4days. | Comparing with the WT and control group (by injecting DEPC water) locust survival rate, we used LT50 to measure dsRNA effect. From the diagram above, CHS dsRNA injection has a great effect to locust which CHS experiment group LT50 is approximately 4days. | ||
| − | + | [[File:T--CAU_China--pw5.jpg|1000px|thumb|center|'''Fig. 2''' LT50 of locust dsRNA injection experiment]] | |
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| − | + | Fig. 2 is the survival rate that we measure during the whole experiment process. | |
Latest revision as of 17:11, 26 October 2020
In our project, we designed three basic part, targeting to three different genes of locust. In our design, we selected Locust migratoria gene trypsin-like serine protease, chitin synthase and ATPase subunitH gene as our target gene. By using specific program to design dsRNA sequence, we finally designed our three basic parts: TSP dsRNA sequence, CHS dsRNA sequence and ATPase dsRNA sequence.
We also measured their effect to locust by injection experiment.
As the result shows:
Comparing with the WT and control group (by injecting DEPC water) locust survival rate, we used LT50 to measure dsRNA effect. From the diagram above, CHS dsRNA injection has a great effect to locust which CHS experiment group LT50 is approximately 4days.
Fig. 2 is the survival rate that we measure during the whole experiment process.