Difference between revisions of "Part:BBa K3599006"
 
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===Overview===
 
===Overview===
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Nitrilase coming from <i>Alcaligenes faecalis</i>, catalysis of the reaction: a nitrile + 2H<sub>2</sub>O = a carboxylate + NH<sub>3</sub>. The codon usage was optimized for expression in <i>Escherichia coli</i>.
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Nitrilase coming from <i>Alcaligenes faecalis</i>, catalysis of the reaction: nitrile + 2H<sub>2</sub>O = carboxylate + NH<sub>3</sub>. The codon usage was optimized for expression in <i>Escherichia coli</i>.
  
  

Latest revision as of 04:11, 27 October 2020


afNIT


Overview

Nitrilase coming from Alcaligenes faecalis, catalysis of the reaction: nitrile + 2H2O = carboxylate + NH3. The codon usage was optimized for expression in Escherichia coli.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 629
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 67
  • 1000
    COMPATIBLE WITH RFC[1000]


Primitive Species Background

A genus of gram-negative, rod-shaped, nonfermenting bacteria (family Achromobacteraceae) that are either motile and peritrichous or nonmotile. They are strictly aerobic; some strains are capable of anaerobic respiration in the presence of nitrate or nitrite; their metabolism is respiratory, never fermentative; they do not use carbohydrates. Found mostly in the intestinal canal, decaying materials, dairy products, water, and soil; they can be isolated from human respiratory and gastrointestinal tracts and wounds in hospitalized patients with compromised immune systems; occasionally the cause of opportunistic infections, including nosocomial septicemia.


Design & Experience

In iGEM2020 TPR_China Project, the coding sequence of afNIT were founded and optimized, further characterization of its enzymatic activity could be done by iGEM teams in the fature.


Reference

Liu, Z. Q. , Dong, L. Z. , Cheng, F. , Xue, Y. P. , Wang, Y. S. , & Ding, J. N. , et al. (2011). Gene cloning, expression, and characterization of a nitrilase from alcaligenes faecalis zjutb10. Journal of Agricultural and Food Chemistry. 10.1021/jf202746a