Difference between revisions of "Part:BBa K115003:Experience"

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===User Reviews===
 
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<I>MattBiggs</I>
 
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Team [http://2011.igem.org/Team:BYU_Provo BYU_Provo 2011] performed random mutagenesis on the <i>Listeria</i> thermosensor and produced six versions of the thermosensor with tighter temperature ranges. This wild type thermosensor acts as a very good positive control that allows translation of reporter product only above 37 deg. C. See the following parts for details:
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[https://parts.igem.org/wiki/index.php?title=Part:BBa_K619890 BBa_K619890],
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[https://parts.igem.org/wiki/index.php?title=Part:BBa_K619891 BBa_K619891],
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[https://parts.igem.org/wiki/index.php?title=Part:BBa_K619892 BBa_K619892],
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[https://parts.igem.org/wiki/index.php?title=Part:BBa_K619893 BBa_K619893],
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[https://parts.igem.org/wiki/index.php?title=Part:BBa_K619894 BBa_K619894],
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[https://parts.igem.org/wiki/index.php?title=Part:BBa_K619895 BBa_K619895],
 
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Latest revision as of 21:26, 27 September 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K115003

The parts were tested in luciferase constructs, by measuring luciferase activity after growing at certain temperatures. The fold increase of luciferase activity between cultures of cells grown at 20ºC and 37ºC is displayed in figure 1.

In this figure, 12 (the leftmost) is construct K115012, the reference strain, which has no thermosensitive RNA, but just B0032 as ribosome binding site. This is to compare for normal temperature induced behaviour in cells. The other constructs from 29-36 are K115029 to K115036, please look at the experience tab of these constructs to see which thermometer RNA they contain.

Figure 1. Fold increase of luminescence per ug of total protein of each construct in respect to luminescence measured at 20ºC. Numbers in the legend represent the last two numbers of the construct name, e.g. 12 = BBa_K115012. Four samples were measured in duplo for every data point. Error bars represent two times SEM.


For more documentation, visit our wiki [http://2008.igem.org/Team:TUDelft (link)].

User Reviews

UNIQ3dacd704fc9ce1aa-partinfo-00000000-QINU

BBa_K115003 MattBiggs

Team [http://2011.igem.org/Team:BYU_Provo BYU_Provo 2011] performed random mutagenesis on the Listeria thermosensor and produced six versions of the thermosensor with tighter temperature ranges. This wild type thermosensor acts as a very good positive control that allows translation of reporter product only above 37 deg. C. See the following parts for details: BBa_K619890, BBa_K619891, BBa_K619892, BBa_K619893, BBa_K619894, BBa_K619895,

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UNIQ3dacd704fc9ce1aa-partinfo-00000002-QINU