Difference between revisions of "Part:BBa B0017"

 
 
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__NOTOC__
 
__NOTOC__
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{|width='80%'
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|-
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|width=35% valign='top'|
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[[Image:Terminator.png]]
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<partinfo>BBa_B0017 parameters</partinfo>
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| width=50% valign='top' style='border: 1px solid black'|
 
<partinfo>BBa_B0017 short</partinfo>
 
<partinfo>BBa_B0017 short</partinfo>
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* Double terminator with two copies of <bbpart>BBa_B0010</bbpart>
  
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Double terminator with two copies of <bbpart>BBa_B0010</bbpart>
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<hr>
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<partinfo>BBa_B0017 SpecifiedComponents</partinfo>
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|}
  
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===Usage and Biology===
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'''Secondary Structure'''
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-- Please enter your experience with this part here --
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<span class='h3bb'>Sequence and Features</span>
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Mfold results with the two copies of BBa_B0010 and the BioBrick scar annotated.
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<partinfo>BBa_B0017 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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[[Image:Mfold-B0017-1.png]]
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<partinfo>BBa_B0017 parameters</partinfo>
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(The top 4 results were similar. The fifth shows the two copies of B0010 with less iteraction.)
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[[Image:Mfold-B0017-5.png]]
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{| width=50% style='border: 2px solid red'
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|-
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|'''Using primers VR/VF2 to PCR B0010 will result in excess bands.'''
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'''See a full description of the problem [[Problems with PCR using VR/VF2|here]].'''
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|-
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|}
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<hr>
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'''Measurement'''
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* [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]

Latest revision as of 20:57, 23 August 2007

Terminator.png

biologyrrnBT1x2
directionForward
forward_efficiency-NA-
reversed_version-NA-
reverse_efficiency-NA-

double terminator (B0010-B0010)

  • Double terminator with two copies of BBa_B0010

B0010
B0010

Secondary Structure

Mfold results with the two copies of BBa_B0010 and the BioBrick scar annotated.

Mfold-B0017-1.png

(The top 4 results were similar. The fifth shows the two copies of B0010 with less iteraction.)

Mfold-B0017-5.png


Using primers VR/VF2 to PCR B0010 will result in excess bands.

See a full description of the problem here.


Measurement

  • [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]