Difference between revisions of "Part:BBa K3396006"

Latest revision as of 18:10, 26 October 2020

GFPnano-Coh2

To make sure that the target GFP protein can connect with TRIM21-DocS, we designed the part which contains Coh2 and GFPnano.

Usage and Biology

Coh2 is a kind of protein which bind with DocS constitutively. GFPnano is the nanobody of GFP, the reporter protein, which can be observed easily. This part can target the GFP protein and connect with TRIM21-DocS and lead to the degradation in a Trim-Away alike method.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 35

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3396006 short</partinfo>
-To make sure that GFPnano-GFP can connect with TRIM21-DocS, we designed the part which bind Coh2 and GFPnano.
+To make sure that the target GFP protein can connect with TRIM21-DocS, we designed the part which contains Coh2 and GFPnano.
 ===Usage and Biology===
-Coh2 is a kind of protein which bind with DocS spontaneously. GFPnano is the nanobody of GFP, a green fluorescent protein, which can be observed easily. This part can target the protein and connect with TRIM21-DocS and lead to the degradation in a Trim-Away alike method. Here is the mechanism of the recombined TRIM21-DocS:
+Coh2 is a kind of protein which bind with DocS constitutively. GFPnano is the nanobody of GFP, the reporter protein, which can be observed easily. This part can target the GFP protein and connect with TRIM21-DocS and lead to the degradation in a Trim-Away alike method.
-.	The GFPnano tagged with Coh2 combines with targeted protein.
-.	TRIM21-DocS connect Coh2-GFPnano-target through the DocS-Coh2 interaction.
-.	The targeted protein is degraded by ubiquitin-proteasome system recruited by TRIM21.
 <span class='h3bb'>Sequence and Features</span>