Difference between revisions of "Part:BBa K3396004"

 
 
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<partinfo>BBa_K3396004 short</partinfo>
 
<partinfo>BBa_K3396004 short</partinfo>
  
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According to the mechanism of the degradation conduced by TRIM21, we designed this Part to make a contrast with FKBP-FKBP-GFPnano.
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This part is designed to generate GFP degradation with improved rapamycin responsiveness.
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
  
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FKBP is a kind of protein which can bind with FRB induced by rapamycin. GFPnano is the nanobody of GFP, a green fluorescent protein, which can be observed easily. This part could target GFP and bind TRIM21-FRB thought the FRB-FKBP interaction and lead to the degradation induced by ubiquitin-proteasome. Here is the mechanism of the recombined TRIM21-FRB:
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1. The GFPnano tagged with FRB combines with targeted protein.
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2. TRIM21-DocS connect Coh2-GFPnano-target through the rapamycin induced DocS-Coh2 interaction.
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3. The targeted protein is degraded by ubiquitin-proteasome system recruited by TRIM21.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3396004 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3396004 SequenceAndFeatures</partinfo>

Latest revision as of 17:48, 26 October 2020


GFPnano-FKBP-FKBP

This part is designed to generate GFP degradation with improved rapamycin responsiveness.


Usage and Biology

FKBP is a kind of protein which can bind with FRB induced by rapamycin. GFPnano is the nanobody of GFP, a green fluorescent protein, which can be observed easily. This part could target GFP and bind TRIM21-FRB thought the FRB-FKBP interaction and lead to the degradation induced by ubiquitin-proteasome. Here is the mechanism of the recombined TRIM21-FRB:

1. The GFPnano tagged with FRB combines with targeted protein.

2. TRIM21-DocS connect Coh2-GFPnano-target through the rapamycin induced DocS-Coh2 interaction.

3. The targeted protein is degraded by ubiquitin-proteasome system recruited by TRIM21.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 35