Difference between revisions of "Part:BBa K3396003"

 
 
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<partinfo>BBa_K3396003 short</partinfo>
 
<partinfo>BBa_K3396003 short</partinfo>
  
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In the condition of our Proof of Concept, we designed the part combined of FKBP and GFPnano.
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This is the fusion protein of GFP nanobody with FKBP on its C terminus.
  
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===Usage and Biology===
 
===Usage and Biology===
  
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FKBP is a kind of protein which can bind with FRB induced by rapamycin. GFPnano is the nanobody of GFP, a green fluorescent protein, which can be observed easily. This part could target GFP and bind TRIM21-FRB thought the FRB-FKBP interaction and lead to the degradation induced by ubiquitin-proteasome.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3396003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3396003 SequenceAndFeatures</partinfo>

Latest revision as of 17:44, 26 October 2020


GFPnano-FKBP

This is the fusion protein of GFP nanobody with FKBP on its C terminus.

Usage and Biology

FKBP is a kind of protein which can bind with FRB induced by rapamycin. GFPnano is the nanobody of GFP, a green fluorescent protein, which can be observed easily. This part could target GFP and bind TRIM21-FRB thought the FRB-FKBP interaction and lead to the degradation induced by ubiquitin-proteasome.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 35