Difference between revisions of "Part:BBa K3384311:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Terminator is a special DNA sequence that is located downstream of the coding region of a gene and can give an RNAP transcription termination signal. We | + | Terminator is a special DNA sequence that is located downstream of the coding region of a gene and can give an RNAP transcription termination signal. We amplified it from the pCAS(#60847). |
| Line 13: | Line 13: | ||
===Source=== | ===Source=== | ||
| − | + | The terminator CYC1 was obtained by PCR amplification using pCas9 plasmid as a template. | |
| − | The terminator CYC1 was obtained by PCR amplification using pCas9 plasmid as a | + | |
===References=== | ===References=== | ||
Latest revision as of 07:40, 27 October 2020
CYC1 terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Terminator is a special DNA sequence that is located downstream of the coding region of a gene and can give an RNAP transcription termination signal. We amplified it from the pCAS(#60847).
Source
The terminator CYC1 was obtained by PCR amplification using pCas9 plasmid as a template.