Difference between revisions of "Part:BBa K3452010"
Howie Chow (Talk | contribs) |
Howie Chow (Talk | contribs) (→Contribution) |
||
| (One intermediate revision by the same user not shown) | |||
| Line 6: | Line 6: | ||
TMCd1 is made up of 156 amino acids, of which 47 are cysteine 2+ residues that can bind 16 Cd. Therefore, Suleman et al. expressed the TMCd1 gene fused to the glutathione S-transferase gene in E. coli BL21 DE3 cells. We used it in the cytoplasm to chelate the cadmium ions inhaled from the cytosol of E. coli. | TMCd1 is made up of 156 amino acids, of which 47 are cysteine 2+ residues that can bind 16 Cd. Therefore, Suleman et al. expressed the TMCd1 gene fused to the glutathione S-transferase gene in E. coli BL21 DE3 cells. We used it in the cytoplasm to chelate the cadmium ions inhaled from the cytosol of E. coli. | ||
| − | === | + | ===Contribution=== |
| − | + | Group: iGEM20_CSU_CHINA (2020-10-26)<br> | |
| + | Author: Hao Zhou<br> | ||
| + | Summary: We have successfully expressed TMCd1 in E. coli DH5α(Figure1 a). The OD600 of E.coli expressed by TMCd1 represents that the concentration of bacteria, will increase faster than that without expression（Figure1. b). <br> | ||
| + | <center>https://2020.igem.org/wiki/images/thumb/a/ae/T--CSU_CHINA--figure-engineering_success.png/800px-T--CSU_CHINA--figure-engineering_success.png</center><br> | ||
| + | <center>Figure1: (a) WB proves the successful expression of our protein, (b) Growth curves of chelated protein (SmtA, hMT-1A, TMCd1)-expressed E. coli, (c) Growth curves of APX2-expressed E. coli, (d) Cadmium uptake curve of (MntH / hMT-1A / MntH & TMCd1 & APX2)-expressed E. coli, (e) Growth curves of ( MntH / hMT-1A / MntH & TMCd1 & APX2)-expressed E. coli.</center><br> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 20:56, 27 October 2020
TMCd1
TMCd1 has a strong cadmium absorbing ability. It's absorption capacity of recombinant strain to Cd increased to 7. 90 μ mol/mg dry cell weight, which was nearly 19 times higher than that of the control strain. TMCd1 is made up of 156 amino acids, of which 47 are cysteine 2+ residues that can bind 16 Cd. Therefore, Suleman et al. expressed the TMCd1 gene fused to the glutathione S-transferase gene in E. coli BL21 DE3 cells. We used it in the cytoplasm to chelate the cadmium ions inhaled from the cytosol of E. coli.
Contribution
Group: iGEM20_CSU_CHINA (2020-10-26)
Author: Hao Zhou
Summary: We have successfully expressed TMCd1 in E. coli DH5α(Figure1 a). The OD600 of E.coli expressed by TMCd1 represents that the concentration of bacteria, will increase faster than that without expression（Figure1. b).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 495
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]