Difference between revisions of "Part:BBa K3606037"
 
Line 9: Line 9:
 
<p>MBP(Maltose Binding Protein) is a protein that normally expressed in the cytoplasm of E.coli. </p>
 
<p>MBP(Maltose Binding Protein) is a protein that normally expressed in the cytoplasm of E.coli. </p>
 
<p>To test the efficiency of our 5 signal peptides for the secretion of the target protein, we fused our 5 signal peptides to MBP to compare their secretion capabilities. The signal peptides and corresponding serial numbers are listed below, through which the biology and characterization of each peptide are available.</p>
 
<p>To test the efficiency of our 5 signal peptides for the secretion of the target protein, we fused our 5 signal peptides to MBP to compare their secretion capabilities. The signal peptides and corresponding serial numbers are listed below, through which the biology and characterization of each peptide are available.</p>
−
NSP4 BBa_K3606042
+
<table>
−
OmpA BBa_K3606043
+
<tr>
−
DsbA BBa_K3606030
+
<th> Signal Peptide </th> <th> Number for Registry </th>
−
PelB BBa_K208004
+
</tr>
−
PhoA BBa_K808028
+
<tr>
 +
<td> NSP4 </td> <td> BBa_K3606042 </td>
 +
</tr>
 +
<tr>
 +
<td> OmpA </td> <td> BBa_K3606043 </td>
 +
</tr>
 +
<tr>
 +
<td> DsbA </td> <td> BBa_K3606030 </td>
 +
</tr>
 +
<tr>
 +
<td> PelB </td> <td> BBa_K208004 </td>
 +
</tr>
 +
<tr>
 +
<td> PhoA </td> <td> BBa_K808028 </td>
 +
</tr>
 +
</table>
  
 
<p>Moreover, we put the expression of secretion peptide guided MBP under the regulation of Ptac, an iPTG inducible promoter, to achieve better qualification of our secretion peptides.</p>
 
<p>Moreover, we put the expression of secretion peptide guided MBP under the regulation of Ptac, an iPTG inducible promoter, to achieve better qualification of our secretion peptides.</p>

Latest revision as of 12:39, 26 October 2020


PlacIq_lacI_Ptac driven Nsp4-MBP

NSP4_MBP is constructed for the test and verification of secretion led by NSP4.

Usage and Biology

MBP(Maltose Binding Protein) is a protein that normally expressed in the cytoplasm of E.coli.

To test the efficiency of our 5 signal peptides for the secretion of the target protein, we fused our 5 signal peptides to MBP to compare their secretion capabilities. The signal peptides and corresponding serial numbers are listed below, through which the biology and characterization of each peptide are available.

Signal Peptide Number for Registry
NSP4 BBa_K3606042
OmpA BBa_K3606043
DsbA BBa_K3606030
PelB BBa_K208004
PhoA BBa_K808028

Moreover, we put the expression of secretion peptide guided MBP under the regulation of Ptac, an iPTG inducible promoter, to achieve better qualification of our secretion peptides.

To test the secretion efficiency of our 5 secretion peptides and compare their secretion capabilities, we also fused them to MBP(Maltose Binding Protein) that normally expressed in the cytoplasm of E.coli. cells. Driven by an iPTG inducible promoter Ptac, we can achieve quantified characterization of these secretion peptides.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1233
    Illegal BglII site found at 1729
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1357
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1427