Difference between revisions of "Part:BBa K3447009"
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The sensing element of AIP. | The sensing element of AIP. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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+ | AgrC and AgrA constitute a two-part system. AgrC is the receptor for AIP. Upon binding to AIP, AgrC dimerizes and phosphorylates each other to activate AgrC proteins. AgrC can phosphorylate AgrA protein. The phosphorylated AgrA protein dimerizes and binds to the P<sub>2</sub> promoter of the agr<sub>BDCA</sub> operon to form a positive feedback loop. The activity of AgrA dimers will also combine with P<sub>3</sub> promoter, to activate the non-coding RNA, the RNA III expression. RNA III can affect the virulence factor and adhesion factor of the translation process.<br> | ||
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+ | [[Image: Diagram of the Agr system of Staphylococcus aureus.jpg|center|frame|100px|<b>Figure 1. </b> Diagram of the Agr system of Staphylococcus aureus]]<br><br> | ||
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+ | In our project, We realize the induction to AIP by constitutive expression of agrA and agrC. The effectiveness of the system was validated by attaching fluorescent proteins downstream of P<sub>2</sub> <partinfo>BBa_K3447011</partinfo>.<br> | ||
+ | AIP induces green fluorescent protein expression: <partinfo>BBa_K3447111</partinfo><br> | ||
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+ | ==<b>Design</b>== | ||
+ | ===Design Notes=== | ||
+ | We added some synonymous mutations to avoid part rules. | ||
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+ | ===Source=== | ||
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+ | We found this sequence data in the reported EMBL/GenBank/DDBJ Nucleotide Sequence Database. | ||
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Latest revision as of 10:53, 27 October 2020
agrA and agrC, an AIP sensing element
The sensing element of AIP.
Usage and Biology
AgrC and AgrA constitute a two-part system. AgrC is the receptor for AIP. Upon binding to AIP, AgrC dimerizes and phosphorylates each other to activate AgrC proteins. AgrC can phosphorylate AgrA protein. The phosphorylated AgrA protein dimerizes and binds to the P2 promoter of the agrBDCA operon to form a positive feedback loop. The activity of AgrA dimers will also combine with P3 promoter, to activate the non-coding RNA, the RNA III expression. RNA III can affect the virulence factor and adhesion factor of the translation process.
In our project, We realize the induction to AIP by constitutive expression of agrA and agrC. The effectiveness of the system was validated by attaching fluorescent proteins downstream of P2 BBa_K3447011.
AIP induces green fluorescent protein expression: BBa_K3447111
Design
Design Notes
We added some synonymous mutations to avoid part rules.
Source
We found this sequence data in the reported EMBL/GenBank/DDBJ Nucleotide Sequence Database.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]