Difference between revisions of "Part:BBa K3657053"
| Line 8: | Line 8: | ||
<p>Lambda N binds to the BoxB RNA hairpin (<a href="https://parts.igem.org/Part:BBa_K3657015">BBa_K3657015</a>). In this way the KRAB protein can be attracted to a specific RNA, for example an RNA that forms a triple helix with DNA or a guide RNA of a dCas. If the RNA binds in front of a promoter, this construct will result in repression of the gene of interest.</p> | <p>Lambda N binds to the BoxB RNA hairpin (<a href="https://parts.igem.org/Part:BBa_K3657015">BBa_K3657015</a>). In this way the KRAB protein can be attracted to a specific RNA, for example an RNA that forms a triple helix with DNA or a guide RNA of a dCas. If the RNA binds in front of a promoter, this construct will result in repression of the gene of interest.</p> | ||
| − | <p>More information here: | + | <p>More information available here: <a href="https://2020.igem.org/Team:Heidelberg/Triple_Helix">iGEM Heidelberg 2020</a></p> |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 00:35, 27 October 2020
Lambda N - KRAB fusion protein
This part is a fusion protein consisting of a viral antiterminal protein lambda N (BBa_K3657014) and a KRAB repressor protein (BBa_K2549055).
Lambda N binds to the BoxB RNA hairpin (BBa_K3657015). In this way the KRAB protein can be attracted to a specific RNA, for example an RNA that forms a triple helix with DNA or a guide RNA of a dCas. If the RNA binds in front of a promoter, this construct will result in repression of the gene of interest.
More information available here: iGEM Heidelberg 2020
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]