Difference between revisions of "Part:BBa K3657053"
 
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This part is a fusion protein consisting of a viral antiterminal protein lambda N (<a href="https://parts.igem.org/Part:BBa_K3657014">BBa_K3657014</a>) and a KRAB repressor protein (<a href="https://parts.igem.org/Part:BBa_K2549055">BBa_K2549055</a>).</p>
 
This part is a fusion protein consisting of a viral antiterminal protein lambda N (<a href="https://parts.igem.org/Part:BBa_K3657014">BBa_K3657014</a>) and a KRAB repressor protein (<a href="https://parts.igem.org/Part:BBa_K2549055">BBa_K2549055</a>).</p>
  
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<p>Lambda N binds to the BoxB RNA hairpin (<a href="https://parts.igem.org/Part:BBa_K3657015">BBa_K3657015</a>). In this way the KRAB protein can be attracted to a specific RNA, for example an RNA that forms a triple helix with DNA or a guide RNA of a dCas. If the RNA binds in proximity of a promoter, this construct will result in repression of the gene of interest.</p>
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<p>Lambda N binds to the BoxB RNA hairpin (<a href="https://parts.igem.org/Part:BBa_K3657015">BBa_K3657015</a>). In this way the KRAB protein can be attracted to a specific RNA, for example an RNA that forms a triple helix with DNA or a guide RNA of a dCas. If the RNA binds in front of a promoter, this construct will result in repression of the gene of interest.</p>
  
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<p>More information here: LINK</p>
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<p>More information available here: <a href="https://2020.igem.org/Team:Heidelberg/Triple_Helix">iGEM Heidelberg 2020</a></p>
  
 
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Latest revision as of 00:35, 27 October 2020


Lambda N - KRAB fusion protein

This part is a fusion protein consisting of a viral antiterminal protein lambda N (BBa_K3657014) and a KRAB repressor protein (BBa_K2549055).

Lambda N binds to the BoxB RNA hairpin (BBa_K3657015). In this way the KRAB protein can be attracted to a specific RNA, for example an RNA that forms a triple helix with DNA or a guide RNA of a dCas. If the RNA binds in front of a promoter, this construct will result in repression of the gene of interest.

More information available here: iGEM Heidelberg 2020

Sequence and Features
Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]