Difference between revisions of "Part:BBa K3657053"

Latest revision as of 00:35, 27 October 2020

Lambda N - KRAB fusion protein

This part is a fusion protein consisting of a viral antiterminal protein lambda N (BBa_K3657014) and a KRAB repressor protein (BBa_K2549055).

Lambda N binds to the BoxB RNA hairpin (BBa_K3657015). In this way the KRAB protein can be attracted to a specific RNA, for example an RNA that forms a triple helix with DNA or a guide RNA of a dCas. If the RNA binds in front of a promoter, this construct will result in repression of the gene of interest.

More information available here: iGEM Heidelberg 2020

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K3657053 short</partinfo>
+{{#tag:html|
+<p>
+This part is a fusion protein consisting of a viral antiterminal protein lambda N (<a href="https://parts.igem.org/Part:BBa_K3657014">BBa_K3657014</a>) and a KRAB repressor protein (<a href="https://parts.igem.org/Part:BBa_K2549055">BBa_K2549055</a>).</p>
-a
+<p>Lambda N binds to the BoxB RNA hairpin (<a href="https://parts.igem.org/Part:BBa_K3657015">BBa_K3657015</a>). In this way the KRAB protein can be attracted to a specific RNA, for example an RNA that forms a triple helix with DNA or a guide RNA of a dCas. If the RNA binds in front of a promoter, this construct will result in repression of the gene of interest.</p>
+<p>More information available here: <a href="https://2020.igem.org/Team:Heidelberg/Triple_Helix">iGEM Heidelberg 2020</a></p>
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 <partinfo>BBa_K3657053 parameters</partinfo>
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+}}