Difference between revisions of "Part:BBa K077557"
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<partinfo>BBa_K077557 short</partinfo> | <partinfo>BBa_K077557 short</partinfo> | ||
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This part constitutively produces LuxR, which forms a complex with HSL from neighboring cells. These LuxR.HSL complexes bind on the cI+LuxR promoter to produce LuxI, responsible for the HSL production to send signals to its own neighbors. Also GFP will be produced to visualize the ON state. | This part constitutively produces LuxR, which forms a complex with HSL from neighboring cells. These LuxR.HSL complexes bind on the cI+LuxR promoter to produce LuxI, responsible for the HSL production to send signals to its own neighbors. Also GFP will be produced to visualize the ON state. | ||
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Input: 3OC6HSL | Input: 3OC6HSL | ||
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Output: LuxR, LuxI (producing 3OC6HSL) and GFP | Output: LuxR, LuxI (producing 3OC6HSL) and GFP | ||
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+ | [[Image:K077557flow.jpg]] | ||
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+ | Flow cytometry results of K077557. A total of 20.000 events were measured. Y-axis indicates number of events. X-axis indicates fluorescence on a logarithmic scale. Z-axis indicates time after induction. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 19:28, 29 October 2008
Part 1 of the Conways game of life system
This part constitutively produces LuxR, which forms a complex with HSL from neighboring cells. These LuxR.HSL complexes bind on the cI+LuxR promoter to produce LuxI, responsible for the HSL production to send signals to its own neighbors. Also GFP will be produced to visualize the ON state.
Input: 3OC6HSL
Output: LuxR, LuxI (producing 3OC6HSL) and GFP
Flow cytometry results of K077557. A total of 20.000 events were measured. Y-axis indicates number of events. X-axis indicates fluorescence on a logarithmic scale. Z-axis indicates time after induction.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1766
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2442