Difference between revisions of "Part:BBa K3657023"
(2 intermediate revisions by the same user not shown) | |||
Line 5: | Line 5: | ||
{{#tag:html| | {{#tag:html| | ||
− | <p>It was proved by Kunkler et al. that this sequence creates a triple helix with the RNA part (<a href= "https://parts.igem.org/Part:BBa_K3657022">BBa_K3657022</a>) <i>in vitro</i>.</p> | + | <p>It was proved by Kunkler et al. that this sequence creates a triple helix with the RNA part (<a href= "https://parts.igem.org/Part:BBa_K3657022">BBa_K3657022</a>) <i>in vitro</i>. (Kunkler et al., 2019)</p> |
− | <p>More information available here: | + | <p>More information available here: <a href="https://2020.igem.org/Team:Heidelberg/Triple_Helix">iGEM Heidelberg 2020</a></p> |
<h4>Usage and Biology</h4> | <h4>Usage and Biology</h4> | ||
<p> Triple helices are one of the alternative structures formed by nucleic acids. In addition to the Watson-Crick interactions between base pairs, Hoogsteen interactions are crucial for the formation of the triple helix. The binding is sequence specific.</p> | <p> Triple helices are one of the alternative structures formed by nucleic acids. In addition to the Watson-Crick interactions between base pairs, Hoogsteen interactions are crucial for the formation of the triple helix. The binding is sequence specific.</p> | ||
<p> | <p> | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
<h4>References</h4> | <h4>References</h4> | ||
<p>Kunkler CN, Hulewicz JP, Hickman SC, Wang MC, McCown PJ, Brown JA. Stability of an RNA•DNA-DNA triple helix depends on base triplet composition and length of the RNA third strand. Nucleic Acids Res. 2019; 47(14):7213-7222.</p> | <p>Kunkler CN, Hulewicz JP, Hickman SC, Wang MC, McCown PJ, Brown JA. Stability of an RNA•DNA-DNA triple helix depends on base triplet composition and length of the RNA third strand. Nucleic Acids Res. 2019; 47(14):7213-7222.</p> |
Latest revision as of 11:02, 27 October 2020
DNA Part of an RNA·DNA-DNA triple helix
It was proved by Kunkler et al. that this sequence creates a triple helix with the RNA part (BBa_K3657022) in vitro. (Kunkler et al., 2019)
More information available here: iGEM Heidelberg 2020
Usage and Biology
Triple helices are one of the alternative structures formed by nucleic acids. In addition to the Watson-Crick interactions between base pairs, Hoogsteen interactions are crucial for the formation of the triple helix. The binding is sequence specific.
References
Kunkler CN, Hulewicz JP, Hickman SC, Wang MC, McCown PJ, Brown JA. Stability of an RNA•DNA-DNA triple helix depends on base triplet composition and length of the RNA third strand. Nucleic Acids Res. 2019; 47(14):7213-7222.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]