Difference between revisions of "Part:BBa K3589151"

Revision as of 16:55, 25 October 2020 (view source) Yanniks (Talk | contribs) ← Older edit		Latest revision as of 19:15, 27 October 2020 (view source) Yanniks (Talk | contribs)
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	<a href="https://parts.igem.org/Part:BBa_K3589150">BBa_K3589150</a> is a variation of this tag which possesses the SP20-portion.		<a href="https://parts.igem.org/Part:BBa_K3589150">BBa_K3589150</a> is a variation of this tag which possesses the SP20-portion.
	</p>		</p>
		+	<br><br>
		+	<p>
		+	<b>Summary of the Results from Team Kaiserslautern 2020 for part BBa_K3589151</b><br>
		+	<ul type=“square“>
		+	<li>We could not detect our secreted protein of interest when combined with this tag, as well as a cCA secretion signal.</li>
		+	</ul>
		+	</p>
		+	<br>
		+
		+	For more information, please visit the registry pages for the L1 and L2 constructs containing this tag:<br>
		+	<p>
		+	<u>L1</u><br>
		+	<a href="https://parts.igem.org/Part:BBa_K3589205">BBa_K3589205</a><br>
		+	<a href="https://parts.igem.org/Part:BBa_K3589206">BBa_K3589206</a><br>
		+	<br>
		+	</p>
		+	<P>
		+	<u>L2</u><br>
		+	<a href="https://parts.igem.org/Part:BBa_K3589211">BBa_K3589211</a><br>
		+	<a href="https://parts.igem.org/Part:BBa_K3589212">BBa_K3589212</a><br>
		+	</p>
		+
		+
		+
		+
	</html>		</html>
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Latest revision as of 19:15, 27 October 2020

HA-RGS-8xHis tag for Chlamydomonas reinhardtii (Phytobrick)

This basic part contains the sequence of the HA-RGS-8His-tag (B5). Combined with a secretion signal of the Kaiser Collection from TU Kaiserslauterns 2019 iGEM Team like BBa_K3002007 (cCA (B2)), BBa_K3002008 (GLE (B2)) or BBa_3002009 (ARS (B2)), an appropriate promoter (BBa_K3002001 (PSAD promoter (A1-B1)) or BBa_K3002031 (PAR promoter (A1-B1))) and a terminator (e.g. BBa_K3002006 (RPL23 terminator)) your target protein should be secreted and you should be able to detect it via HA-antibody and purify it via a nickel-NTA column.
Note that this part misses a SP20-portion. The SP20-portion is reported to improve the secretion of proteins by increasing the glycosylation (see Team TU Kaiserslautern 2019's results). It is a repetition of Prolin and Serin, whereby Prolin is converted into hydroxyproline and then glycolyzed (Ramos-Martinez et. al, 2017).

BBa_K3589150 is a variation of this tag which possesses the SP20-portion.

Summary of the Results from Team Kaiserslautern 2020 for part BBa_K3589151

• We could not detect our secreted protein of interest when combined with this tag, as well as a cCA secretion signal.

For more information, please visit the registry pages for the L1 and L2 constructs containing this tag:

L1
BBa_K3589205
BBa_K3589206

L2
BBa_K3589211
BBa_K3589212

Sequence and Features