Difference between revisions of "Part:BBa K3589150"
 
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<partinfo>BBa_K3589150 short</partinfo>
 
<partinfo>BBa_K3589150 short</partinfo>
  
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This basic part contains the sequence of the SP20-HA-RGS-8His-tag (B5). Combined with a secretion signal of the Kaiser Collection from TU Kaiserslauterns 2019 iGEM Team like BBa_K3002007 (cCA (B2)), BBa_BBa_K3002008 (GLE (B2)) or BBa_3002009 (ARS (B2)), an appropriate promoter (BBa_K3002001 (PSAD promoter (A1-B1)) or BBa_K3002031 (PAR promoter (A1-B1))) and a terminator (e.g. BBa_BBa_K3002006(RPL23 terminator)) your target protein should be secreted efficiently and you should be able to detect it via HA-antibody and purify it via a nickel-NTA column.
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<html>
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The SP20 tag should improve the secretion of proteins by increasing the glycosylation. It is a repetition of Prolin and Serin, whereby Prolin is converted into hydroxyproline and then glycolyzed.
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<p>
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This basic part contains the sequence of the SP20-HA-RGS-8His-tag (B5). Combined with a secretion signal of the Kaiser Collection from TU Kaiserslauterns 2019 iGEM Team like <a href="https://parts.igem.org/Part:BBa_K3002007">BBa_K3002007</a> (cCA (B2)), <a href="https://parts.igem.org/Part:BBa_K3002008">BBa_K3002008</a> (GLE (B2)) or <a href="https://parts.igem.org/Part:BBa_K3002009">BBa_3002009</a> (ARS (B2)), an appropriate promoter (<a href="https://parts.igem.org/Part:BBa_K3002001">BBa_K3002001 </a>(PSAD promoter (A1-B1)) or <a href="https://parts.igem.org/Part:BBa_K3002031">BBa_K3002031</a> (PAR promoter (A1-B1))) and a terminator (e.g. <a href="https://parts.igem.org/Part:BBa_K3002006">BBa_K3002006</a> (RPL23 terminator)) your target protein should be secreted efficiently and you should be able to detect it via HA-antibody and purify it via a nickel-NTA column.<br>
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The SP20 tag should improve the secretion of proteins by increasing the glycosylation. It is a repetition of Prolin and Serin, whereby Prolin is converted into hydroxyproline and then glycolyzed (<a href="https://doi.org/10.1111/pbi.12710">Ramos-Martinez <I>et. al</I>, 2017</a>).
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</p>
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<br><br>
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<p>
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<b>Summary of the Results from Team Kaiserslautern 2020 for part BBa_K3589150</b><br>
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<ul type=“square“>
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<li>We could not detect our secreted protein of interest when combined with this tag, as well as a cCA secretion signal.</li>
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</ul>
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</p>
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<br>
  
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For more information, please visit the registry pages for the L1 and L2 constructs containing this tag:<br>
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<p>
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<u>L1</u><br>
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<a href="https://parts.igem.org/Part:BBa_K3589203">BBa_K3589203</a><br>
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<a href="https://parts.igem.org/Part:BBa_K3589204">BBa_K3589204</a><br>
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<br>
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</p>
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<P>
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<u>L2</u><br>
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<a href="https://parts.igem.org/Part:BBa_K3589209">BBa_K3589209</a><br>
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<a href="https://parts.igem.org/Part:BBa_K3589210">BBa_K3589210</a><br>
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</p>
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</html>
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 19:13, 27 October 2020


SP20-HA-RGS-8xHis tag for Chlamydomonas reinhardtii (Phytobrick)

This basic part contains the sequence of the SP20-HA-RGS-8His-tag (B5). Combined with a secretion signal of the Kaiser Collection from TU Kaiserslauterns 2019 iGEM Team like BBa_K3002007 (cCA (B2)), BBa_K3002008 (GLE (B2)) or BBa_3002009 (ARS (B2)), an appropriate promoter (BBa_K3002001 (PSAD promoter (A1-B1)) or BBa_K3002031 (PAR promoter (A1-B1))) and a terminator (e.g. BBa_K3002006 (RPL23 terminator)) your target protein should be secreted efficiently and you should be able to detect it via HA-antibody and purify it via a nickel-NTA column.
The SP20 tag should improve the secretion of proteins by increasing the glycosylation. It is a repetition of Prolin and Serin, whereby Prolin is converted into hydroxyproline and then glycolyzed (Ramos-Martinez et. al, 2017).



Summary of the Results from Team Kaiserslautern 2020 for part BBa_K3589150

  • We could not detect our secreted protein of interest when combined with this tag, as well as a cCA secretion signal.


For more information, please visit the registry pages for the L1 and L2 constructs containing this tag:

L1
BBa_K3589203
BBa_K3589204

L2
BBa_K3589209
BBa_K3589210

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]