Difference between revisions of "Part:BBa K128003"
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from MIT iGEM 2008: this research team has shown p1025 inhibiting binding to hydroxyapatite (synthetic teeth) through a "novel tooth binding assay". The following graph shows the S. Mutans that are not bound to hydroxyapatite cell count. When p1025 was present, there were more cells unbound in t1, meaning that fewer cells bound to the hydroxyapatite. | from MIT iGEM 2008: this research team has shown p1025 inhibiting binding to hydroxyapatite (synthetic teeth) through a "novel tooth binding assay". The following graph shows the S. Mutans that are not bound to hydroxyapatite cell count. When p1025 was present, there were more cells unbound in t1, meaning that fewer cells bound to the hydroxyapatite. | ||
| − | For more about the "novel toothbinding assay": | + | For more about the "novel toothbinding assay": [http://2008.igem.org/Team:MIT/Tooth_binding_assay_protocol Tooth Binding Assay] |
[[Image:p1025.png| 300px| Variable plate layout]] | [[Image:p1025.png| 300px| Variable plate layout]] | ||
Latest revision as of 18:50, 29 October 2008
p1025
This sequence codes for a short peptide, found to competitively inhibit binding of S.mutans to the tooth surface (CG et al.; Nature Biotechnol. 1999). S.mutans takes in sugars and secretes lactic acid, causing dental cavities, so introduction of this peptide into the mouth prevents cavities. This part uses the modified Silver BioBrick prefix and suffix to allow for protein construction.
from MIT iGEM 2008: this research team has shown p1025 inhibiting binding to hydroxyapatite (synthetic teeth) through a "novel tooth binding assay". The following graph shows the S. Mutans that are not bound to hydroxyapatite cell count. When p1025 was present, there were more cells unbound in t1, meaning that fewer cells bound to the hydroxyapatite.
For more about the "novel toothbinding assay": [http://2008.igem.org/Team:MIT/Tooth_binding_assay_protocol Tooth Binding Assay]
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal suffix found in sequence at 81
Illegal EcoRI site found at 1
Illegal XbaI site found at 16 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal SpeI site found at 82
Illegal PstI site found at 96
Illegal NotI site found at 7
Illegal NotI site found at 89 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 82 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal XbaI site found at 16
Illegal SpeI site found at 82
Illegal PstI site found at 96
Illegal NgoMIV site found at 42 - 1000COMPATIBLE WITH RFC[1000]