Difference between revisions of "Part:BBa K3396013"

 
 
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This is the XTEN Linker gene flanked with specific restriction sites that could be recognized by BsmBI.
 
This is the XTEN Linker gene flanked with specific restriction sites that could be recognized by BsmBI.
  
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===Usage and Biology===
 
===Usage and Biology===
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This part was designed by us to add replace modules in the PREDATOR Pro system conveniently. Two BsmBI sites of were added to the 5' and 3’ end of XTEN Linker, through which, other coding sequences can be inserted using Golden-Gate Assembly.
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<html>
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<p><img src="https://static.igem.org/mediawiki/parts/c/c1/T--NUDT_CHINA--Repla-1.png" alt="" width="500" /></p>
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</html>
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Figure 1. Schematic representation of the Replaceable-1.
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===Sequence and Features===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3396013 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3396013 SequenceAndFeatures</partinfo>

Latest revision as of 20:27, 27 October 2020


Repleceable-1

This is the XTEN Linker gene flanked with specific restriction sites that could be recognized by BsmBI.

Usage and Biology

This part was designed by us to add replace modules in the PREDATOR Pro system conveniently. Two BsmBI sites of were added to the 5' and 3’ end of XTEN Linker, through which, other coding sequences can be inserted using Golden-Gate Assembly.


Figure 1. Schematic representation of the Replaceable-1.

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]