Difference between revisions of "Part:BBa K3589209:Design"

 
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===Design Notes===
 
===Design Notes===
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This part is compatible with the MoClo standard and codon optimized for <i>C. reinhardtii.</i>
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===Source===
 
===Source===
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This part was created using the MoClo system.
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===References===
 
===References===

Latest revision as of 01:46, 28 October 2020


Level 2 spectinomycin resistance + cCA_BaLac_SP20-HA-RGS-8His


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2401
    Illegal PstI site found at 3586
    Illegal PstI site found at 3673
    Illegal PstI site found at 3737
    Illegal PstI site found at 4109
    Illegal PstI site found at 4397
    Illegal PstI site found at 4474
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2401
    Illegal NheI site found at 2665
    Illegal PstI site found at 3586
    Illegal PstI site found at 3673
    Illegal PstI site found at 3737
    Illegal PstI site found at 4109
    Illegal PstI site found at 4397
    Illegal PstI site found at 4474
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2401
    Illegal BglII site found at 4033
    Illegal BglII site found at 4992
    Illegal BamHI site found at 4372
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2401
    Illegal PstI site found at 3586
    Illegal PstI site found at 3673
    Illegal PstI site found at 3737
    Illegal PstI site found at 4109
    Illegal PstI site found at 4397
    Illegal PstI site found at 4474
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2401
    Illegal PstI site found at 3586
    Illegal PstI site found at 3673
    Illegal PstI site found at 3737
    Illegal PstI site found at 4109
    Illegal PstI site found at 4397
    Illegal PstI site found at 4474
    Illegal NgoMIV site found at 1401
    Illegal NgoMIV site found at 1584
    Illegal NgoMIV site found at 1694
    Illegal NgoMIV site found at 6232
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is compatible with the MoClo standard and codon optimized for C. reinhardtii.

Source

This part was created using the MoClo system.

References