Difference between revisions of "Part:BBa K3380603:Design"

(→‎References)
(→‎Design Notes)
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
This construct has not been tested in the lab, however the Edinburgh iGEM 2020 team tested the BBa_K3380500 construct. The BBa_K3380603 construct can be assembled following a similar protocol (on the experience page of the BBA_K3380500 construct).
 
  
 
All the sequences for the individual parts were acquired. The individual part were designed with 4bp scars to allow swapping of the parts to test different constructs. The individual parts were phosphorylated, annealed and ligated together using the T4 ligase.
 
All the sequences for the individual parts were acquired. The individual part were designed with 4bp scars to allow swapping of the parts to test different constructs. The individual parts were phosphorylated, annealed and ligated together using the T4 ligase.

Latest revision as of 10:34, 25 October 2020


TXO ArsR regulated construct under T7 promoter (BBa_K190015) expressing iSpinach


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

All the sequences for the individual parts were acquired. The individual part were designed with 4bp scars to allow swapping of the parts to test different constructs. The individual parts were phosphorylated, annealed and ligated together using the T4 ligase.

Source

The parts were purchased as de novo synthesized sequences.

References

Millacura, F.A., Li, M., Valenzuela-Ortega, M.A. and French, C., 2019. TXO: Transcription-Only genetic circuits as a novel cell-free approach for Synthetic Biology. bioRxiv, p.826230